Nadogradnja će biti dostupna 30. listopada (točno za Haloween) putem Steama. Planirana je i za ''druge platforme'', vjerojatno za PS4, PS Vitu, Wii U, 3DS te Xbox One; međutim, nije sigurno da li će se izlazak za konzole moći ostvariti na gore spomenuti datum.
The Binding of Isaac: Afterbirth sveukupno donosi preko 100 sati sadržaja. Sve novitete možete pročitati u priloženom, originalnom, zapisniku.
- Greed Mode: a whole new game mode with a heavy focus on risk/reward that is a completely new way to play that features new endings and a final boss.
- Expanded main game: a new final area has been added to the main game along with another new final boss and ending.
- Daily Runs: compete for highscore / best time on the leader boards in these daily seeded runs.
- Ten totally new and awesome challenges
- 1000+ new rooms, as well as new tiny rooms, closets and giant arching hallways
- Totally new playable character, Lilith, mother of demons
- 120 new items, not counting tons of new pickups, chests, pills, bombs, cards etc. taking the item count up beyond 525
- Four new alternate chapters with new chapter specific enemy types visual themes and surprises
- New seed code combo page, as well as a butt ton of new seed codes that mod the game
- Expanded soundtrack
- New room types like, trap rooms, double treasure rooms and ultra rare closets
- Greatly expanded and updated HUD system
- Tons of new secret transformations
- Eight new bosses
- 25+ new enemies
- Updated weapon combo system
- Far too many new achievements
- 20 million new co-op babies